Bactericidal activity of multiple combinations of tigecycline and colistin against NDM-1-producing Enterobacteriaceae
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Updated: May 24, 2026

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
Published on: April 18, 2019
Mahableshwar Albur1, Alan Noel, Karen Bowker
1Bristol Centre for Antimicrobial Research and Evaluation, Department of Medical Microbiology, North Bristol NHS Trust, Southmead Hospital, Westbury-on-Trym, Bristol, United Kingdom. msalbur@hotmail.com
Abstract:
The interaction between colistin and tigecycline against eight well-characterized NDM-1-producing Enterobacteriaceae strains was studied. Time-kill methodology was employed using a 4-by-4 exposure matrix with pharmacokinetically achievable free drug peak, trough, and average 24-h serum concentrations. Colistin sulfate and methanesulfonate alone showed good early bactericidal activity, often with subsequent regrowth. Tigecycline alone had poor activity. Addition of tigecycline to colistin does not produce increased bacterial killing; instead, it may cause antagonism at lower concentrations.
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