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A human neoplastic hematopoietic cell line producing a fibroblast type of interferon
Developments in Biological Standardization
|January 1, 1979
Abstract:
1301 is a subline of the EBV-genome negative T leukemia line CCRF-CEM. IF was induced by Sendai virus. By use of neutralizing antisera and by studies of some physical properties it could be shown that IF was of the fibroblast type. This was confirmed by its antiviral effect on heterologous cell lines.
Insights
Researchers induced interferon (IF) in a T leukemia cell line using Sendai virus. They identified this IF as fibroblast-type, confirming its antiviral properties against other cell lines.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- The study utilizes the 1301 cell line, a subline of the EBV-genome negative T leukemia line CCRF-CEM.
- Interferon (IF) induction was investigated in this specific cell line.
Purpose of the Study:
- To characterize the type of interferon induced in the 1301 cell line.
- To confirm the antiviral activity of the induced interferon.
Main Methods:
- Induction of interferon (IF) using Sendai virus.
- Characterization of IF using neutralizing antisera.
- Analysis of physical properties of the induced IF.
- Assessment of antiviral effects on heterologous cell lines.
Main Results:
- Interferon (IF) was successfully induced in the 1301 cell line.
- Neutralizing antisera and physical property studies indicated the IF was of the fibroblast type.
- The induced fibroblast-type IF demonstrated antiviral effects on heterologous cell lines.
Conclusions:
- The 1301 cell line can produce fibroblast-type interferon upon stimulation with Sendai virus.
- The induced interferon exhibits functional antiviral activity, validating its classification.