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Related Experiment Videos

Transmission electron microscopy of previously embedded celloidin sections.

D Portmann1, J Fayad, F H Linthicum

  • 1House Ear Institute, Los Angeles, California.

Acta Oto-Laryngologica. Supplementum
|January 1, 1990
PubMed
Summary

A new method re-embeds celloidin sections of human temporal bones for transmission electron microscopy (TEM). This technique enables detailed analysis of the inner ear

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Area of Science:

  • Otolaryngology
  • Histology
  • Microscopy

Background:

  • Celloidin embedding is a common method for preserving human temporal bones.
  • Transmission electron microscopy (TEM) requires specific sample preparation for high-resolution imaging.
  • Analyzing the delicate structures of the inner ear using TEM presents significant challenges.

Purpose of the Study:

  • To present a novel technique for re-embedding celloidin-embedded human temporal bone sections for TEM analysis.
  • To enable high-resolution ultrastructural examination of the intact human inner ear.
  • To facilitate retrospective studies on existing temporal bone collections.

Main Methods:

  • A four-step procedure involving loosening sections, removing celloidin with clove oil, staining, and epoxy resin embedding.

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  • Utilizing transmission electron microscopy for ultrastructural analysis.
  • Preserving the integrity of the membranous labyrinth without prior dissection.

    • Main Results:

    • Successful re-embedding of celloidin sections for TEM.
    • TEM analysis of intact labyrinth in various regions of the same specimen and section.
    • Preservation of tissue integrity allowing for detailed ultrastructural examination.

    Conclusions:

    • The presented technique offers a valuable method for TEM analysis of human temporal bone sections.
    • This approach simplifies the study of the inner ear's ultrastructure, eliminating the need for fragile tissue dissection.
    • The method supports retrospective studies and the investigation of ultrastructural histopathology in optimally preserved human inner ear tissue.