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Updated: May 24, 2026
High-Density Lipoprotein-Specific Phospholipid Efflux Assay
Published on: September 30, 2025
Analysis of in vitro oxidized human LDL phospholipids by solid-phase extraction and micellar electrokinetic capillary
Ying-Ling Yang1, Chin-Pong Chong, Ming-Hua Tsai
1Department of Chemistry, National Changhua University of Education, Changhua, Taiwan.
Abstract:
Phospholipids of in vitro oxidized human low-density lipoproteins (LDL) were separated by two different solid-phase extraction (SPE) methods. One of the two methods was designed to test the effects of gradient elution. This SPE method isolated more phospholipids from in vitro oxidized LDL than the other one according to the results of liquid chromatography and electrospray ionization mass spectrometry (LC ESI-MS) analysis. A micellar electrokinetic capillary chromatography (MEKC) method was also used to analyze phospholipids separated by SPE. The results of MEKC and LC ESI-MS were consistent for the major phospholipid classes, including PC, lysoPC, PE, PI and PS. The MEKC profiles showed significant differences for native and oxidized LDL phospholipids. Therefore, the unique combination of SPE and MEKC methods showed dramatic distinctions between native and in vitro oxidized human LDL phospholipids. The combination also shows great potential for rapid analysis of in vivo oxidized human LDL phospholipids in the future.
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