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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

Published on: December 5, 2014

Data processing for 3D mass spectrometry imaging.

Xingchuang Xiong1, Wei Xu, Livia S Eberlin

  • 1School of Life Science, Beijing Institute of Technology, Beijing, China.

Journal of the American Society for Mass Spectrometry
|March 7, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces novel data processing methods for three-dimensional mass spectrometry (3D-MS) imaging, enhancing tissue analysis. The developed software solutions improve 3D-MS imaging visualization and statistical analysis for biological samples.

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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
08:14

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
08:16

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Published on: January 16, 2018

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Computational Biology

Background:

  • Three-dimensional mass spectrometry (3D-MS) imaging presents significant data processing challenges.
  • Practical implementation requires addressing data reduction, alignment, visualization, and statistical analysis of tissue sections.

Purpose of the Study:

  • To investigate and develop computational solutions for 3D-MS imaging data processing.
  • To establish a robust methodology for analyzing complex 3D tissue data.

Main Methods:

  • Utilized MATLAB functions for developing software solutions.
  • Implemented peak detection and alignment for data reduction and mass accuracy.
  • Employed classification methods for tissue section alignment and 3D data set construction.

Main Results:

  • Successfully reduced data size while maintaining mass accuracy.
  • Developed a method for aligning 2D imaging data based on tissue morphology.
  • Constructed a 3D dataset enabling 3D visualization and analysis.

Conclusions:

  • The developed methodology effectively addresses key challenges in 3D-MS imaging data processing.
  • The approach enables enhanced visualization and statistical analysis of 3D tissue data.
  • Demonstrated the utility of the methodology using desorption electrospray ionization mass spectrometry (DESI-MS) imaging of a mouse brain.