[Determination of 5-hydroxytryptamine in plasma by nanofiber solid phase extraction-high performance liquid...

Area of Science:

• Analytical Chemistry
• Biochemistry

Context:

• Accurate quantification of 5-hydroxytryptamine (5-HT) is crucial for understanding neurological and physiological processes.
• Existing methods for 5-HT determination in plasma can be complex or lack sufficient sensitivity.

Purpose:

• To develop and validate a novel, sensitive, and reproducible method for determining 5-hydroxytryptamine (5-HT) in human plasma.
• To utilize packed-nanofiber solid phase extraction coupled with high-performance liquid chromatography-electrochemical detection (HPLC-ECD) for enhanced analysis.

Summary:

• A packed-nanofiber solid phase extraction (SPE) technique was coupled with high-performance liquid chromatography-electrochemical detection (HPLC-ECD) for 5-HT analysis.
• Protein precipitation, derivatization with o-phthalaldehyde (OPA), and SPE purification were employed.
• The method demonstrated a wide linear range (5–500 µg/L), low limit of detection (1 µg/L), high recovery (95.6%–101.4%), and good reproducibility (RSD < 5%).

Impact:

• This validated method provides a simple, highly sensitive, and reproducible approach for 5-HT determination in human plasma.
• The technique has potential applications in clinical diagnostics and biomedical research requiring accurate 5-HT level monitoring.