Effect of Streptococcus sanguinis/Porphyromonas gingivalis single and combined biofilms upon platelet aggregation

To assess the effect of two oral bacteria Streptococcus sanguinis and Porphyromonas gingivalis upon platelet aggregation.

Streptococcus sanguinis, P. gingivalis, S. sanguniis+P. gingivalis were added to platelet-rich plasma and platelet aggregation measured using a platelet aggregometer. Platelets were passed through a flow chamber with S. sanguinis, P. gingivalis or a biofilm of S. sanguinis and P. gingivalis coated with saliva. Platelet adhesion to the chamber was observed under a fluorescence microscope for 15min. The positive control was platelets treated with adrenaline; the negative control was platelets treated with phosphate-buffered saline.

The mean (± s.e.) aggregation magnitude of S. sanguinis and P. gingivalis was 77.7±7.4% and 79.3±9.9%, respectively. The aggregation magnitude of S. sanguinis+P. gingivalis was 51.3±12.9%, which was significantly lower than that for S. sanguinis/P. gingivalis (P<0.05). In the flow chamber system, platelets adhered to S. sanguinis/P.gingivalis respectively within 3min, and reached a plateau at 5-15min. Under the condition of the S. sanguinis- and P. gingivalis-saliva biofilm, platelet adhesion to the biofilm was significantly reduced at 5-15min (P<0.05).

In the static or dynamic flow system, platelets adhered to S. sanguinis or P. gingivalis. However, if S. sanguinis was mixed with P. gingivalis, the aggregation magnitude (%) was significantly reduced.