Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Room-Temperature Luminescence of Eosin Y and Phloxine B in Red- to Near-Infrared Optical Region.

Journal of fluorescence·2026
Same author

Luminescence of N<sup>2</sup>,3-etheno-2-aminopurine Embedded in Polyvinyl Alcohol Films at Room Temperature.

Luminescence : the journal of biological and chemical luminescence·2026
Same author

Room temperature luminescence of a triangulenium dye ADOTA in PVA films.

Methods and applications in fluorescence·2026
Same author

Spectral properties of quinine sulfate in PVA films for front-face format emission measurements.

Methods and applications in fluorescence·2026
Same author

Advancing Dry Electroencephalography With Scalable, Soft, and Transcranial Magnetic Stimulation-Compatible Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene Electrodes for Research and Clinical-Grade Applications.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Biochemical clocks in fingerprints energy transfer between free tryptophan and NADH for potential estimation of postmortem interval, age, and sex.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same journal

Bioactivity and Bioavailability of Tea (Camelia sinensis) and Its Possible Effect on Disease Prevention and Health Promotion: A Narrative Review.

Current pharmaceutical biotechnology·2026
Same journal

Therapeutic Applications and Advancements of Exosome-Based Treatments - A Comprehensive Review.

Current pharmaceutical biotechnology·2026
Same journal

Comparative Phytochemical Characterization and Antibacterial Activity of Ethanol and Aqueous Extracts of Moringa peregrina and Moringa oleifera against Multidrug-Resistant Bacteria.

Current pharmaceutical biotechnology·2026
Same journal

Unveiling Aptamers for Targeted Tumour Therapies and Detection: Systematic Evolution of Ligands by Exponential Enrichment (SELEX) Technology in Oncology.

Current pharmaceutical biotechnology·2026
Same journal

Nanosuspensions-Based Dry Powder Inhalers for Pulmonary Delivery of Hydrophobic Natural Products: Formulation Strategies, Efficacy, and Challenges.

Current pharmaceutical biotechnology·2026
Same journal

Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Accelerate Diabetic Rat Wound Healing by Inhibiting Pyroptosis through the NLRP3/Caspase-1/GSDMD Pathway.

Current pharmaceutical biotechnology·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

FRET enhanced fluorescent nanodiamonds.

Rafal Fudala, Sangram Raut, Badri P Maliwal

  • 1Department of Physics and Astronomy, Texas Christian University, TCU Box 298840, Fort Worth, TX 76129, USA. zgryczynski@tcu.edu.

Current Pharmaceutical Biotechnology
|March 8, 2012
PubMed
Summary
This summary is machine-generated.

Fluorescent nanodiamonds (FNDs) show enhanced brightness using Forster resonance energy transfer (FRET). Organic dyes harvest light and transfer energy to FNDs for improved imaging and drug delivery applications.

More Related Videos

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Related Experiment Videos

Last Updated: May 24, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Materials Science

Background:

  • Fluorescent nanodiamonds (FNDs) are promising biocompatible nanomaterials for imaging and drug delivery.
  • FNDs possess excellent photostability and fluorescence properties but suffer from low brightness due to limited absorption.
  • The (N-V)(-) centers in FNDs have low absorption cross-sections, hindering their signal intensity.

Purpose of the Study:

  • To significantly enhance the fluorescence signal of single nanodiamonds.
  • To explore the use of Forster resonance energy transfer (FRET) for improving FND brightness.
  • To develop brighter FND-based theranostic agents.

Main Methods:

  • Utilizing Forster resonance energy transfer (FRET) to couple organic dyes (fluorophores) with FNDs.
  • Attaching multiple organic dyes to the FND surface to act as light-harvesting antennas.
  • Employing functional groups on the FND surface for direct dye linkage or dendrimer-based dye positioning.
  • Leveraging remaining functional groups for particle targeting and drug delivery.

Main Results:

  • Demonstrated efficient energy transfer from attached organic dyes to the (N-V)(-) centers in FNDs.
  • Showcased significant enhancement of the fluorescence signal from individual nanodiamonds via FRET.
  • Confirmed the potential for creating ultrahigh brightness FNDs.

Conclusions:

  • FRET-based approaches can overcome the brightness limitations of FNDs.
  • Functionalized FNDs with enhanced fluorescence offer a new platform for theranostic applications.
  • This method enables the design of highly bright, photostable nanodiamonds for targeted drug delivery and imaging.