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Updated: May 24, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
[Preparation and characterization of monoclonal antibody against Vibrio vulnificus]
Kai-fei Fu1, Cong Ma, Jian-wei Guo
1Clinical Laboratory, Navy General Hospital, Beijing, China. fukaifei@sina.com
Aim:
To prepare high-performance and specific monoclonal antibody (mAb) against Vibrio vulnificus and carry out characterization.
Methods:
BALB/c mice were immunized with Vibrio vulnificus protein, and hybridoma cells against Vibrio vulnificus were produced by cellsion technique. The titers of mAbs against vvhA and cross-reaction between the anti-vvhA mAb and other other important marine bacteria were screened by ELISA and Western blot.
Results:
Five strains of hybridoma were obtained. Identification result indicated that 5 mAbs have favourable specifity and immunoreactivity.
Conclusion:
Specific mAbs against Vibrio vulnificus were produced which provides an important preparation for establishing rapid-detection kit of detecting Vibrio vulnificus.

