Related Experiment Video
Updated: May 24, 2026
11:21
Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers
Published on: August 30, 2024
Intracellular conformations of human telomeric quadruplexes studied by electron paramagnetic resonance spectroscopy
Mykhailo Azarkh1, Vijay Singh, Oliver Okle
1Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, Konstanz, Germany.
Abstract:
In-cell DEER (in-cell double electron electron resonance) is applied to investigate quadruplex formation of the human telomeric repeat d[AGGG(TTAGGG)(3)]. The initially unfolded DNA sequence forms a mixture of different quadruplex topologies upon injection into living cells. In addition, time-dependent distance measurements are carried out to monitor quadruplex folding.
Related Concept Videos
02:41Telomeres and Telomerase
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
02:31Replication in Eukaryotes
Overview