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NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

NF-kB-dependent Signaling Pathway

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

Aging

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...

The Effect of Aging on Tissues

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...

Co-activators and Co-repressors

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

Mitochondria

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Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

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Related Experiment Video

Updated: May 24, 2026

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

NF-κB in Aging and Disease.

Jeremy S Tilstra¹, Cheryl L Clauson, Laura J Niedernhofer

¹Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Aging and Disease

|March 8, 2012

Summary

Cellular damage activates stress responses, including the IKK/NF-κB pathway, which drives aging. Inhibiting this pathway may extend mammalian healthspan by delaying age-related diseases.

Keywords:

Aging Inflammation NF-κB Senescence

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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

Area of Science:

Cellular and Molecular Biology
Aging Research
Signaling Pathways

Background:

Stochastic damage to cellular components is a primary aging driver.
Stress response pathways, particularly IKK/NF-κB signaling, are implicated in aging.
NF-κB activation is linked to age-related diseases like Alzheimer's, diabetes, and osteoporosis.

Purpose of the Study:

To investigate the role of the IKK/NF-κB signaling pathway in the aging process.
To explore NF-κB as a potential therapeutic target for extending healthspan.

Main Methods:

Review of existing literature on cellular damage, stress responses, and aging.
Analysis of the IKK/NF-κB pathway's activation by various stresses.
Examination of NF-κB's role in age-related pathologies and lifespan regulation.

Main Results:

NF-κB pathway is activated by genotoxic, oxidative, and inflammatory stresses.
Increased NF-κB transcriptional activity is observed in aging tissues.
Inhibition of NF-κB in mouse models delays age-related symptoms and pathologies.
NF-κB activation is interconnected with known lifespan regulators (insulin/IGF-1, FOXO, SIRT, mTOR, DNA damage).

Conclusions:

The IKK/NF-κB pathway is a key mediator of aging and age-related diseases.
Targeting NF-κB offers a promising therapeutic strategy for extending mammalian healthspan.