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Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis

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Dynamics of subcellular proteomes during brain development.

Daniel B McClatchy¹, Lujian Liao, Ji Hyoung Lee

¹The Scripps Research Institute, La Jolla, California 92037, United States.

Journal of Proteome Research

|March 9, 2012

Summary

This study offers the most comprehensive quantitative analysis of the developing brain proteome to date. It identifies over 5000 significant developmental changes, including novel neurodevelopment regulators, aiding in understanding neurological disorders.

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Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

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Published on: May 21, 2019

Area of Science:

Neuroscience
Proteomics
Developmental Biology

Background:

Neurological disorders often stem from disruptions in brain development.
Early identification of developmental perturbations requires a detailed understanding of the brain's developmental process.

Purpose of the Study:

To provide a comprehensive quantitative proteomic analysis of the developing rat brain.
To identify significant proteomic changes during brain development and discover novel regulators of neurodevelopment.

Main Methods:

Mass spectrometry was used to analyze synaptosomal and mitochondrial fractions from three rat brain regions at four postnatal time points.
Stable Isotope Labeling in Mammals (SILAM) with (15)N-labeled rats was employed for quantitative analysis.
Over 167,000 peptides were quantified, identifying more than 5,000 significant changes.

Main Results:

Over 5,000 statistically significant protein changes were identified during brain development, including proteins associated with known neurological diseases.
Distinct proteomic trends were observed between synaptic and non-synaptic mitochondrial fractions.
Common protein networks across different brain regions were identified, each with unique expression patterns.
Novel regulators of neurodevelopment exhibiting temporal patterns similar to known regulators were discovered.

Conclusions:

This study presents the most extensive quantitative proteomic dataset of the developing brain to date.
The findings provide a valuable resource for neurobiologists studying brain development and neurological disorders.
The identification of novel neurodevelopment regulators opens new avenues for research into the etiology of neurological conditions.