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Updated: May 24, 2026
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury
Published on: June 17, 2020
A comprehensive protein expression profile of extracellular matrix biomaterial derived from porcine urinary bladder
Helder Marçal1, Tania Ahmed, Stephen F Badylak
1Bio/Polymer Research Group, Centre for Advanced Macromolecular Design, School of Biotechnology & Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Aims:
To generate a comprehensive profile of the protein composition of xenogeneic biomaterial, derived from porcine urinary bladder matrix (UBM).
Materials & Methods:
Tunica layers and muscularis mucosa were removed from bladders using mechanical delamination. UBM was prepared using a solution of peracetic acid in ethanol, lyophilized then milled into powder. UBM biomaterial was subjected to tryptic digests and components separated using high-performance liquid chromatography with an ion trap mass spectrometer and identified through databases.
Results:
A repertoire of 129 proteins with neurotrophic, antiangiogenic and tumor-suppressive activities and those associated with tissue remodeling and wound repair were identified.
Conclusion:
This study provides the first insight into the complex nature of the UBM and how its application may be tailored for specific applications in regenerative medicine. We propose that the UBM be further investigated for reconstructive and regenerative remodeling of cardiac and dermal tissues, as well as peripheral nerves.
