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Updated: May 24, 2026
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
Published on: October 24, 2018
Characterization of the phenotype and functionality of corneal epithelial cells derived from mouse embryonic stem
Maria Notara1, Diana Hernandez, Christopher Mason
1Department of Ocular Biology & Therapeutics, UCL Institute of Ophthalmology, London, UK. m.notara@ucl.ac.uk
Aims:
To investigate the optimum conditions for the differentiation of a mouse embryonic stem cell line towards corneal epithelial cell fate.
Materials & Methods:
The effect of conditioned media from both metabolically active (to produce lineage A) and growth-arrested limbal fibroblasts (lineage G) were compared with basal media (lineage N) in terms of morphology and marker expression, assessed by immunocytochemistry and reverse transcription PCR. Cultures were transplanted into a porcine ex vivo model to investigate their ability for wound healing and cornea repair.
Results:
Lineage N exhibited cobblestone morphology and expressed CK12 and p63α, while OCT4 and SSEA1 were downregulated. Post-transplantation, these cells were able to multilayer and heal after wounding while maintaining marker expression.
Conclusion:
Lineages with corneal epithelial-like characteristics, which are derived from embryonic stem cells, have potential for use in the study of corneal wound healing and therapy.
