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Related Experiment Video

Updated: May 24, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Translation initiation factor eIF4E is a target for tumor cell radiosensitization.

Thomas J Hayman¹, Eli S Williams, Muhammad Jamal

¹University of South Florida Morsani College of Medicine, Tampa, Florida, USA.

Cancer Research

|March 9, 2012

Summary

Targeting eukaryotic initiation factor 4E (eIF4E) enhances tumor cell radiosensitivity. Inhibiting eIF4E increases cancer cell susceptibility to radiation, offering a potential therapeutic strategy.

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Published on: May 19, 2014

Area of Science:

Molecular Biology
Cancer Research
Radiation Oncology

Background:

Translational control of gene expression is crucial for cellular response to radiation.
Eukaryotic initiation factor 4E (eIF4E) selectively enhances cap-dependent mRNA translation and is a key translation regulator.

Purpose of the Study:

To investigate the regulatory role of eIF4E in cellular radiosensitivity.
To determine if targeting eIF4E can enhance tumor cell susceptibility to radiation therapy.

Main Methods:

Silencing of eIF4E in tumor and normal cell lines.
Pharmacologic inhibition of eIF4E using ribavirin.
Analysis of cell-cycle distribution, apoptosis, γH2AX foci dispersion, and mitotic catastrophe.
Assessment of eIF4E binding to transcripts post-radiation.

Main Results:

eIF4E silencing and ribavirin treatment significantly enhanced tumor cell radiosensitivity, but not that of normal cells.
eIF4E attenuation delayed γH2AX foci dispersion and increased mitotic catastrophe following radiation.
Radiation increased eIF4E binding to over 1,000 transcripts involved in DNA replication, recombination, and repair.

Conclusions:

eIF4E plays a significant role in regulating tumor cell radiosensitivity.
Targeting eIF4E represents a promising therapeutic strategy to improve the efficacy of radiation therapy in cancer treatment.