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Related Experiment Video

Updated: May 24, 2026

Extracellular Vesicle Tissue Factor Activity Assay

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Tissue factor-driven thrombin generation and inflammation in atherosclerosis.

Hugo ten Cate¹

¹Laboratory for Clinical Thrombosis and Hemostasis, Department of Internal medicine and Cardiovascular Research Institute Maastricht (CARIM), Maastricht University Medical Center, Maastricht, The Netherlands. h.tencate@maastrichtuniversity.nl

Thrombosis Research

|March 9, 2012

Summary

Tissue factor and local thrombin generation play roles in atherosclerosis development. Anticoagulant interventions targeting these pathways show promise in reducing disease progression and inflammation in mouse models.

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Area of Science:

Cardiovascular Biology
Hematology
Pathophysiology

Background:

Tissue factor (TF) is a transmembrane receptor present in atherosclerotic lesions.
Local thrombin and fibrin generation may influence cellular functions in atherogenesis, including inflammation and cell proliferation.

Purpose of the Study:

To investigate the role of the coagulation system, particularly tissue factor and thrombin, in atherosclerosis.
To evaluate the impact of anticoagulant interventions on atherosclerosis progression and associated inflammation in experimental models.

Main Methods:

Studies in apoE(-/-) mice to assess atherosclerosis.
Evaluation of hypercoagulability and plaque phenotype.
Interventions included modulating tissue factor pathway inhibitor, and administration of selective factor Xa and thrombin inhibitors (rivaroxaban, dabigatran).

Main Results:

Hypercoagulability correlates with increased atherosclerosis in mice.
Overexpression of tissue factor pathway inhibitor reduced thrombogenicity and neo intima formation.
Selective inhibition of factor Xa and thrombin reduced inflammation in atherosclerotic mice.

Conclusions:

The local coagulation machinery, involving tissue factor and thrombin, is implicated in atherosclerosis.
Novel selective anticoagulants demonstrate potential in modifying atherosclerosis and associated inflammation.
Further clinical studies are warranted to explore the therapeutic potential of these agents in atherosclerosis.