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Measuring platelet aggregation with microplate reader. A new technical approach to platelet aggregation studies.

J C Fratantoni1, B J Poindexter

  • 1Division of Blood and Blood Products, Center for Biologics Evaluation and Research, Bethesda, Maryland 20892.

American Journal of Clinical Pathology
|November 1, 1990
PubMed
Summary

This study presents a novel microtiter plate method for measuring platelet aggregation, offering a rapid and simultaneous analysis of multiple samples. This technique provides reliable results comparable to standard aggregometers.

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Area of Science:

  • Hematology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Platelet aggregation is crucial for hemostasis and thrombosis.
  • Accurate measurement of platelet aggregation is essential for diagnosing and managing bleeding and clotting disorders.
  • Existing methods can be time-consuming and require specialized equipment.

Purpose of the Study:

  • To develop and validate a modified microtiter plate reader method for quantifying platelet aggregation.
  • To establish a rapid, high-throughput assay for platelet function.
  • To compare the performance of the microtiter plate method with a conventional aggregometer.

Main Methods:

  • Utilized a commercially available microtiter plate reader with a modified horizontal agitation system (1.3 mm amplitude, 1,360 cycles/minute).

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  • Performed simultaneous analysis of multiple platelet samples (test and control) in 96-well plates.
  • Validated the technique by comparing dose-response curves with standard aggregometry using adenosine diphosphate, thrombin, and arachidonic acid as agonists.

    • Main Results:

    • Achieved satisfactory platelet aggregation curves using the modified microtiter plate reader.
    • Obtained data within a rapid 10-15 minute timeframe.
    • Demonstrated high similarity in dose-response curves between the microtiter plate technique and standard aggregometry.

    Conclusions:

    • The modified microtiter plate reader method is a valid and efficient technique for measuring platelet aggregation.
    • This assay allows for simultaneous, high-throughput analysis of platelet function.
    • The method offers a reliable alternative to traditional aggregometers for research and diagnostic applications.