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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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Related Experiment Video

Updated: May 24, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Selective isolation of G-quadruplexes by affinity chromatography.

Tianjun Chang1, Xiangjun Liu, Xiaohong Cheng

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Journal of Chromatography. A
|March 9, 2012
PubMed
Summary

Researchers developed affinity matrices to isolate G-quadruplexes (G4s), a key nucleic acid structure. A Pery01-based matrix effectively isolated parallel G4s from plasma, aiding G4 structure studies.

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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

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Last Updated: May 24, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

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In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • G-quadruplexes (G4s) are distinctive secondary structures in nucleic acids with repetitive guanine sequences.
  • Bioinformatics analysis reveals G4-forming sequences are widespread in the human genome.
  • G4s are implicated in numerous crucial biological processes.

Purpose of the Study:

  • To develop effective affinity matrices for the selective isolation of G-quadruplexes.
  • To investigate the interactions governing G4 binding to affinity matrices.
  • To establish a method for directly isolating G4 structures from biological samples.

Main Methods:

  • Preparation of four affinity matrices utilizing hemin or a perylene derivative (Pery01) as ligands.
  • Investigation of the retention behaviors of various G4 structures on the prepared matrices.
  • Characterization of the role of π-π stacking and electrostatic interactions in G4 isolation.

Main Results:

  • The π-π stacking interaction between the ligand and the G-tetrad is critical for selective G4 isolation.
  • Electrostatic interactions between DNA and the matrix contribute to nonspecific binding.
  • A Pery01-immobilized matrix on PGMA beads demonstrated high selectivity for parallel G4 structures.

Conclusions:

  • Affinity matrices, particularly those employing Pery01, can selectively isolate G-quadruplexes based on structural features.
  • The developed Pery01-based matrix successfully isolated a spiked parallel G4 directly from plasma.
  • This method provides a valuable tool for studying G4 structures and their biological roles.