Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polyphenols extracted from Lycium barbarum seeds regulate gut microbiota to improve type 2 diabetes mellitus in db/db mice.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Unlocking the functional potential of broccoli protein: A comparative study of deep eutectic solvent and traditional extraction based on proteomics.

International journal of biological macromolecules·2026
Same author

Dietary ginsenosides for longevity: from biosynthesis and bioactive potential to functional food applications.

Critical reviews in food science and nutrition·2026
Same author

A Phenome-Wide Association and Mendelian Randomization Study of Genetically Predicted Membranous Nephropathy with Multiple Diseases.

Kidney & blood pressure research·2026
Same author

Analysis on the difference of nutrient components of black wolfberry seed grown in different regions.

Food research international (Ottawa, Ont.)·2026
Same author

Unlocking the anti-aging potential of Lycium barbarum polysaccharides: Structure-activity insights, mechanisms, and future therapeutics.

International journal of biological macromolecules·2026

Related Experiment Video

Updated: May 24, 2026

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
04:39

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter

Published on: June 13, 2025

Polypyrrole hollow fiber for solid phase extraction.

Tian Tian1, Jianjun Deng, Zhuoying Xie

  • 1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, P. R. China.

The Analyst
|March 9, 2012
PubMed
Summary

Researchers created conductive polypyrrole hollow fibers for solid-phase extraction. This novel method efficiently extracts neuroendocrine markers from human plasma, showing high recovery rates for behavioral disorder analysis.

More Related Videos

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
10:34

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Published on: August 3, 2016

Related Experiment Videos

Last Updated: May 24, 2026

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
04:39

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter

Published on: June 13, 2025

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
13:35

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
10:34

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water

Published on: August 3, 2016

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Solid-phase extraction (SPE) is crucial for analyzing complex biological samples.
  • Developing efficient and selective sorbents is key to improving SPE methods.
  • Neuroendocrine markers are important biomarkers for behavioral disorders.

Purpose of the Study:

  • To develop a novel solid-phase extraction method using conductive polypyrrole hollow fibers.
  • To evaluate the efficacy of these fibers for extracting polar neuroendocrine markers from human plasma.
  • To assess the potential of this method for quantitative analysis in complex matrices.

Main Methods:

  • Fabrication of conductive polypyrrole (PPy) hollow fibers via electrospinning of poly(e-caprolactone) (PCL) templates followed by in situ polymerization.
  • Utilizing PCL templates to create burr-shaped or smooth PPy fiber shells, subsequently removed to yield hollow fibers.
  • Application of the hollow PPy fibers as sorbents for solid-phase extraction of 5-hydroxyindole-3-acetic acid and homovanillic acid from human plasma.

Main Results:

  • Hollow PPy fibers demonstrated effective multi-functionality as ideal sorbents for polar compounds.
  • Successful extraction of two key neuroendocrine markers, 5-hydroxyindole-3-acetic acid and homovanillic acid, from human plasma.
  • Achieved high absolute recovery rates of 90.7% for 5-hydroxyindole-3-acetic acid and 92.4% for homovanillic acid under optimized conditions.

Conclusions:

  • The developed method using conductive polypyrrole hollow fibers offers a simple, selective, and sensitive approach for solid-phase extraction.
  • This technique shows significant potential for the quantitative analysis of polar species in complex biological samples.
  • The method provides a promising tool for analyzing neuroendocrine markers related to behavioral disorders.