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Proteomic analysis of lamellar bodies isolated from amniotic fluid: implications for function
Ross Ridsdale1, David F Lewis, Timothy E Weaver
1Department of Pediatrics, Division of Neonatology, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
American Journal of Perinatology
|March 9, 2012
Summary
Lamellar bodies (LBs) in amniotic fluid, used for fetal lung maturity assessment, were analyzed. These LBs contain proteins involved in host defense and anti-inflammatory functions, suggesting lung origin and a role in fetal innate immunity.
Area of Science:
- Biochemistry
- Pulmonology
- Neonatalogy
Background:
- Lamellar body count (LBC) in amniotic fluid assesses fetal lung maturity.
- The origin and function of amniotic fluid lamellar bodies (LBs) remain uncharacterized.
Purpose of the Study:
- To identify proteins within amniotic fluid LBs.
- To investigate the biogenesis and potential functions of amniotic fluid LBs.
Main Methods:
- Purification of LBs from term gestation amniotic fluid with mature LBC.
- Proteomic analysis using tandem mass spectrometry to identify LB proteins.
Main Results:
- Identified 122 unique proteins in amniotic fluid LBs.
- Found >40% concordance with rat lung LB proteome, but minimal overlap with human epidermal LBs.
- Identified proteins predominantly associated with host defense and anti-inflammatory functions.
Conclusions:
- Amniotic fluid LBs are likely derived from fetal lung secretions.
- These LBs may contribute to the fetus's innate host defense mechanisms.
