Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Quantitative Electrostatic Potential Descriptor Enables Deep Learning-Accelerated Discovery of High-Performance Lithium-Ion Battery Electrolytes.

Angewandte Chemie (International ed. in English)·2026

Same author

Molecular recognition and cross scale mechanisms for selective uranium recovery from seawater.

Water research·2026

Same author

The ATF2-KNSTRN Axis Harnesses Lactate to Modulate MLH1/MSH2 Expression and Gemcitabine Resistance in Lung Adenocarcinoma.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same author

Enzyme-assisted catalytic hairpin assembly based aggregation-induced emission assay for trace miRNA-196a in serum.

The Analyst·2026

Same author

CALR-dependent endoplasmic reticulum N-glycoprotein maturation promotes Japanese encephalitis virus entry and replication.

Cell & bioscience·2026

Same author

Insect-Cell-Produced AAV2 Vectors Activate Myeloid Innate Immune Response Featuring NETosis.

Molecular pharmaceutics·2026

Search research articles

Related Experiment Video

Updated: May 24, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Design strategies for aptamer-based biosensors.

Kun Han¹, Zhiqiang Liang, Nandi Zhou

¹Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China. hank@sibet.ac.cn

Sensors (Basel, Switzerland)

|March 9, 2012

Summary

Aptamers are versatile recognition elements for biosensor development, offering advantages over antibodies. This review details four aptamer-based biosensor design strategies for detecting various targets.

Keywords:

aptamer biosensor design strategy

More Related Videos

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: May 24, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Biotechnology
Analytical Chemistry
Molecular Recognition

Background:

Aptamers are increasingly utilized as recognition elements in biosensor construction.
They serve as promising alternatives to antibodies for detecting protein and small molecule targets.
Aptamer-based biosensors offer significant advantages in bioassay applications.

Purpose of the Study:

To provide a comprehensive overview of aptamer-based biosensor design strategies.
To classify existing aptamer biosensor fabrication methods into distinct operational modes.
To highlight the potential of aptamer biosensors in future bioassay development.

Main Methods:

Review of literature on aptamer-based biosensor design and fabrication.
Classification of biosensor strategies into four fundamental modes: target-induced structure switching, sandwich/sandwich-like, target-induced dissociation/displacement, and competitive replacement.
Analysis of the advantages and applications of each design strategy.

Main Results:

Aptamers are effective recognition elements for biosensors.
Four primary design strategies for aptamer-based biosensors have been identified and classified.
Each strategy leverages unique aptamer properties for target detection.

Conclusions:

Aptamer-based biosensors represent a powerful tool for bioassays.
Smart design strategies enhance the capabilities of aptamer biosensors.
These biosensors are poised to become leading devices in bioassay-related fields.