Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which forms a...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
Coagulation01:09

Coagulation

The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prothrombotic fibrin clot phenotype in obstetric antiphospholipid syndrome: clinical implications and unanswered questions. Authors' reply.

Polish archives of internal medicine·2026
Same author

Altered fibrin clot properties and the risk of thromboembolism in women with obstetric antiphospholipid syndrome: a cohort study.

Polish archives of internal medicine·2025
Same author

Beyond prothrombin time and activated partial thromboplastin time: coagulation in vivo-an illustrated review.

Laboratory medicine·2025
Same author

Elevated carbonylated proteins are associated with major cardiovascular events in patients with chronic coronary syndrome: A cohort study.

Kardiologia polska·2024
Same author

Elevated factor XIa as a modulator of plasma fibrin clot properties in coronary artery disease.

European journal of clinical investigation·2023
Same author

Anti-FVIII antibodies in Black and White hemophilia A subjects: do F8 haplotypes play a role?

Blood advances·2022
Same journal

Apoptotic versus procoagulant platelets: similar "necrotic" phenotype and procoagulant activity in vitro, but distinct adhesive protein composition.

Thrombosis research·2026
Same journal

Heatstroke-induced coagulopathy: A scoping review of therapeutic strategies and outcome reporting.

Thrombosis research·2026
Same journal

Mapping thrombus habitat: Non-contrast MRI radiomics and pixel-tile histomics approach to track venous thrombosis evolution in mice.

Thrombosis research·2026
Same journal

A study protocol for a randomised controlled trial evaluating the safety and efficiency of the YEARS algorithm versus computed tomography pulmonary angiography only for suspected acute pulmonary embolism in patients with cancer: the Hydra Study.

Thrombosis research·2026
Same journal

Associating the phenotypic expression of platelets with disease type through image-based single-cell profiling.

Thrombosis research·2026
Same journal

The mechanisms of contractile dysfunction following chronic limited platelet activation in (pro)thrombotic conditions.

Thrombosis research·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Decryption of tissue factor.

Saulius Butenas1, Jolanta Krudysz-Amblo

  • 1University of Vermont, Department of Biochemistry, Burlington, VT 05446, USA. sbutenas@uvm.edu

Thrombosis Research
|March 10, 2012
PubMed
Summary
This summary is machine-generated.

The role of the Cys(186)-Cys(209) disulfide bond in cell surface tissue factor (TF) activity remains unclear. Current research suggests this bond

More Related Videos

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Related Experiment Videos

Last Updated: May 24, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Hematology

Background:

  • Tissue factor (TF) initiates blood coagulation via complex formation with factor VIIa (FVIIa).
  • Cell-surface TF activity is debated, with theories suggesting it's either encrypted or requires specific activation.
  • The Cys(186)-Cys(209) disulfide bond is proposed to be crucial for TF activity, but its role is controversial.

Purpose of the Study:

  • To investigate the precise role of the Cys(186)-Cys(209) disulfide bond in cell-surface TF activity.
  • To address the controversy surrounding TF decryption and activation mechanisms.

Main Methods:

  • Analysis of TF epitopes and critical amino acids involved in TF/FVIIa complex formation.
  • Evaluation of cell membrane lipid composition's influence on TF activity.
  • Investigating the impact of the Cys(186)-Cys(209) disulfide bond on TF function.

Main Results:

  • Existing studies show conflicting results regarding the Cys(186)-Cys(209) disulfide bond's role in TF activity.
  • Lack of specificity in reagents used for cell treatment may contribute to controversial findings.
  • The exact influence of this disulfide bond on cell-surface TF function remains undetermined.

Conclusions:

  • The contribution of the Cys(186)-Cys(209) disulfide bond to cell-surface TF activity is not definitively established.
  • Further research with highly specific reagents is needed to clarify TF decryption and activation pathways.