Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robot-Assisted Adaptive Control Enhances Dental Drilling Force Stability.

International dental journal·2026
Same author

Amelioration of neurogenic erectile dysfunction by ST36 electroacupuncture.

iScience·2026
Same author

Autonomous robotic execution of nonlinear toolpaths for geometry-matched osteotomy in tooth autotransplantation: an in vitro study.

International journal of oral science·2026
Same author

Relatively upstream factors associated with pressure injury occurrence and staging in older hospitalized patients: a prospective observational study.

BMC geriatrics·2026
Same author

Autonomous robotic post space preparation and immediate ceramic post-and-core restoration of a maxillary right canine: A clinical report.

The Journal of prosthetic dentistry·2026
Same author

ApoEVs@FeSe<sub>2</sub>-RGD as a synergistic redox-immune regulator for accelerating wound healing.

Journal of materials chemistry. B·2026

Related Experiment Video

Updated: May 24, 2026

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

Increased fibroblast functionality on CNN2-loaded titania nanotubes.

Hongbo Wei1, Shuyi Wu, Zhihong Feng

  • 1Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, People's Republic of China.

International Journal of Nanomedicine
|March 10, 2012
PubMed
Summary

This study developed titania nanotubes loaded with connective tissue growth factor (CCN2) to improve fibroblast function. This innovation aims to enhance soft tissue seals around percutaneous implants, reducing infection and implant failure.

Keywords:
adhesionanodizationconnective tissue growth factorfibroblasttitania nanotubes

More Related Videos

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

Related Experiment Videos

Last Updated: May 24, 2026

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
07:14

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Published on: May 10, 2020

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Percutaneous implants face challenges like infection and epithelial downgrowth due to poor implant-skin interface closure.
  • Fibroblasts are crucial for forming stable soft tissue biologic seals around implant sites.

Purpose of the Study:

  • To develop a novel drug delivery system using titania nanotubes loaded with connective tissue growth factor (CCN2) to enhance fibroblast functionality.
  • To investigate the potential of CCN2-loaded titania nanotubes in promoting stable soft tissue biologic seals for maxillofacial implants.

Main Methods:

  • Fabrication of titanium implants with anodized titania nanotubes loaded with an 11.2 kDa C-terminal CCN2 fragment.
  • Utilized a simplified lyophilization method for CCN2 loading with approximately 80% efficiency.
  • Investigated CCN2 release kinetics and evaluated fibroblast adhesion, viability, and actin cytoskeleton organization in vitro.

Main Results:

  • CCN2-loaded titania nanotubes demonstrated significantly increased fibroblast adhesion over 24 hours.
  • Enhanced fibroblast viability was observed for up to 5 days.
  • Improved actin cytoskeleton organization was noted on surfaces with CCN2-loaded titania nanotubes compared to controls.

Conclusions:

  • CCN2-loaded titania nanotubes effectively enhance fibroblast functionality in vitro.
  • This approach shows promise for developing improved percutaneous implants that promote stable soft tissue biologic seals.
  • Further in vivo studies are warranted to validate these findings for clinical applications.