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Related Experiment Videos

Identification of a mislabeled fixed specimen by DNA analysis.

D Shibata1, T Namiki, R Higuchi

  • 1Department of Pathology, Los Angeles County-University of Southern California Medical Center 90033.

The American Journal of Surgical Pathology
|November 1, 1990
PubMed
Summary

Genotypic analysis of formalin-fixed tissues accurately identifies surgical specimens using DNA. This molecular genetic method distinguishes between fixed tissues, aiding in specimen identification and preventing mislabeling.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Histopathology

Background:

  • Formalin-fixed, paraffin-embedded (FFPE) surgical specimens are crucial for diagnosis but can be subject to misidentification.
  • Histological examination alone may not always provide definitive specimen identification.
  • Genotypic analysis offers a potential method for unambiguous specimen identification.

Purpose of the Study:

  • To evaluate the utility of molecular genetic analysis for identifying formalin-fixed, paraffin-embedded surgical specimens.
  • To demonstrate the capability of DNA analysis to distinguish between different tissue samples.
  • To report a case where genotypic analysis identified a mislabeled specimen.

Main Methods:

  • DNA extraction from single sections of FFPE tissue.

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  • Polymerase chain reaction (PCR) amplification of the human leukocyte antigen (HLA) DQ alpha and low-density lipoprotein receptor genes.
  • Genotyping using a non-isotopic color detection method for HLA DQ alpha.

    • Main Results:

    • Successful DNA extraction and amplification from FFPE tissues.
    • Polymorphic genetic markers (HLA DQ alpha and LDL receptor) allowed for differentiation between specimens.
    • A case of a mislabeled mastectomy specimen was identified due to identical genotypes.
    • The molecular genetic analysis was completed within 2 days.

    Conclusions:

    • Molecular genetic analysis of DNA from FFPE specimens provides reliable genotypic identification.
    • This technique can distinguish between fixed tissues based on allelic differences.
    • Genotypic analysis offers valuable information beyond traditional histology, particularly for identifying mislabeled specimens.