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Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

Charting the NF-κB pathway interactome map.

Paolo Tieri1, Alberto Termanini, Elena Bellavista

  • 1CIG Luigi Galvani Interdept Center, University of Bologna, Bologna, Italy. p.tieri@iac.cnr.it

Plos One
|March 10, 2012
PubMed
Summary
This summary is machine-generated.

The Nuclear Factor κB (NF-κB) signaling system, crucial for inflammation, involves more proteins than previously thought. This study reveals a complex NF-κB interactome with central, overlooked proteins influencing inflammatory responses.

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Area of Science:

  • Molecular Biology
  • Immunology
  • Systems Biology

Background:

  • Inflammation is a key physiological response mediated by the Nuclear Factor κB (NF-κB) signaling pathway.
  • Canonical representations of NF-κB signaling may overlook crucial components influencing its complex regulatory network.

Purpose of the Study:

  • To reconstruct a comprehensive NF-κB interactome by integrating diverse data sources.
  • To provide a systemic view of the NF-κB signaling network beyond canonical pathway models.

Main Methods:

  • Gathering and curating data from literature, functional enrichment resources, and pathway databases.
  • Analyzing protein-protein interactions to map the NF-κB activation system.
  • Reconstructing a comprehensive NF-κB interactome.

Main Results:

  • The reconstructed NF-κB interactome is significantly larger and more complex than canonical representations.
  • Several topologically central proteins, previously neglected, play critical roles in NF-κB activation.
  • The NF-κB interactome exhibits a bow tie architecture.

Conclusions:

  • Canonical pathway representations underestimate the complexity of the NF-κB signaling system.
  • A broader understanding of the NF-κB interactome is essential for comprehending inflammatory responses.
  • The developed interactome serves as an open resource for future research into NF-κB-mediated processes.