[Preparation for and study on the property of medical bacterial cellulose]

Bacterial cellulose (BC) was prepared by Acetobacter xylinum in static culture. After purified by chemical treatment, the microstructure, chemical structure, crystal structure and mechanical property of BC were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD) and tensile strength measurement respectively, and compared with those of the imported bacterial cellulose wound dressing served as control sample (XBC). The results indicated that the diameter of the BC was (22 +/- 9) nm, and the crystallinity index was 89.71%. The tensile strength and the Young's mouduls of BC were significant higher than XBC both in wet and dry states. The biocompatibility of BC and XBC were evaluated by cytotoxicity test, delayed contact sensitization study in the Guinea Pig and skin irritation test. The results showed that BC had reliable biocompatibility as well as XBC. With the unique nanostructure, high crystallinity, high mechanical strength, and reliable biocompatibility, BC produced in our country as well as XBC can be used as a safe biomaterial for the medical applications.