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Related Experiment Video

Updated: May 24, 2026

Quantitative Immunofluorescence to Measure Global Localized Translation
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Quantitative Immunofluorescence to Measure Global Localized Translation

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Quantitative imaging with fluorescent biosensors.

Sakiko Okumoto1, Alexander Jones, Wolf B Frommer

  • 1Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA..

Annual Review of Plant Biology
|March 13, 2012
PubMed
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Novel biosensors offer precise subcellular measurements of molecular dynamics, overcoming limitations of traditional methods like mass spectrometry and promoter-based reporters for real-time cellular analysis.

Area of Science:

  • Cellular and Molecular Biology
  • Biophysics
  • Biotechnology

Background:

  • Cellular processes are dynamic and vary spatially, necessitating tools for quantitative analysis.
  • Existing methods like mass spectrometry and promoter reporters have limited temporal and spatial resolution.
  • Understanding cellular and subcellular dynamics is crucial for organismal function.

Purpose of the Study:

  • To review novel biosensor tools for quantifying molecular dynamics at subcellular resolution.
  • To provide a comprehensive database of sensors for diverse analytes and processes.
  • To illustrate applications of these sensors in live cell imaging.

Main Methods:

  • Development and application of translocation sensors.
  • Utilization of fluorescence-intensity sensors.

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Glutamine Flux Imaging Using Genetically Encoded Sensors
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Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

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Last Updated: May 24, 2026

Quantitative Immunofluorescence to Measure Global Localized Translation
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Published on: August 22, 2017

Glutamine Flux Imaging Using Genetically Encoded Sensors
10:23

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

  • Employing Förster resonance energy transfer (FRET) sensors.

    • Main Results:

    • A comprehensive database of over 70 sensors for various analytes and processes is presented.
    • Examples of quantitative live cell imaging applications are provided.
    • Novel tools enable direct quantification of enzyme activities, protein dynamics, and biophysical processes.

    Conclusions:

    • Biosensors provide essential tools for high-resolution, real-time monitoring of molecular dynamics within cells.
    • These advanced tools overcome limitations of conventional techniques, enabling deeper biological insights.
    • The reviewed sensors facilitate quantitative live cell imaging and functional understanding.