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Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Published on: June 29, 2020
A rapid and highly reliable field-based LAMP assay of canine parvovirus
M Parthiban1, K C Divya, K Kumanan
1Department of Animal Biotechnology, Madras Veterinary College, Chennai, India. drparthiban66@gmail.com
Unlabelled:
Loop-mediated isothermal amplification (LAMP) is known as a rapid and reliable alternative to conventional single-step or nested PCR for detection of genomic DNA of various pathogens in clinical samples. In this study, LAMP assay was developed for canine parvovirus (CPV) and compared with single-step and nested PCR assays. Out of 50 fecal samples from dogs clinically suspected for CPV infections, 19 were found positive by single-step PCR, 22 by nested PCR and 26 by LAMP. LAMP products were subjected to restriction analysis and sequencing to check their specificity. LAMP assay turned out to be a rapid and fairly reproducible method, did not amplify other common canine pathogens and was more sensitive than nested PCR assay. Therefore, it can be regarded as a highly reliable method for routine field diagnosis of CPV infection.
Keywords:
canine parvovirus; nested polymerase chain reaction; loop-mediated isothermal amplification; sensitivity; specificity.
