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Coagulation

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The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
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Updated: May 24, 2026

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

Published on: August 4, 2018

Imaging coagulation reactions in vivo.

Lacramioara Ivanciu¹, Sriram Krishnaswamy, Rodney M Camire

¹Department of Pediatrics, Division of Hematology, The Children's Hospital of Philadelphia, PA, USA.

Thrombosis Research

|March 13, 2012

Summary

Advanced videomicroscopy reveals new insights into blood clot formation, challenging long-held theories derived from in vitro studies. This technology offers a sensitive approach to understanding hemostasis in vivo.

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Area of Science:

Hematology
Vascular Biology
Biophysics

Background:

Understanding blood coagulation and thrombus formation in vivo is incomplete.
Existing knowledge gaps stem from limitations in molecular tools and analytical sensitivity.
In vitro studies have shaped current, potentially outdated, concepts of hemostasis.

Purpose of the Study:

To review state-of-the-art videomicroscopy for imaging hemostasis in vivo.
To highlight novel insights into early hemostatic events provided by this technology.
To discuss the limitations and future directions of in vivo hemostasis research.

Main Methods:

Utilizing advanced live-cell videomicroscopy.
Inducing laser injury to mouse cremaster arterioles.
In vivo imaging of hemostasis and thrombus formation.

Main Results:

Videomicroscopy provides high-resolution insights into in vivo hemostasis.
New data challenges established concepts of coagulation enzyme complex function.
The technology facilitates a deeper understanding of blood cell and vasculature interactions.

Conclusions:

In vivo videomicroscopy is crucial for advancing hemostasis research.
This technology offers a more accurate reflection of physiological thrombus formation than in vitro models.
Further development and application of these methods will refine our understanding of coagulation.