Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Phase II Reactions: Glucuronidation01:24

Phase II Reactions: Glucuronidation

Glucuronidation, a pivotal phase II biotransformation process, involves the coupling of glucuronic acid to a drug or xenobiotic. Given its widespread occurrence and critical role in drug metabolism, it's considered the most crucial phase II reaction. It enhances the water solubility of substances, aiding their expulsion from the body. The driving force behind these reactions is a group of enzymes known as UDP-glucuronosyltransferases (UGTs). UGTs facilitate the transfer of a glucuronic acid...
Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Convenient Route to Large-Scale Chemical Synthesis of <i>p</i>-Hydroxyphenylacetaldehyde Oxime and Its <i>p</i>-β-d-Glucopyranoside: Key Intermediates and Products in Plant Specialized Metabolism.

ACS omega·2024
Same author

Essentials in the acquisition, interpretation, and reporting of plant metabolite profiles.

Phytochemistry·2024
Same author

Phytoalexins of the crucifer Barbarea vulgaris: Structural profile and correlation with glucosinolate turnover.

Phytochemistry·2023
Same author

Recruitment of distinct UDP-glycosyltransferase families demonstrates dynamic evolution of chemical defense within Eucalyptus L'Hér.

The New phytologist·2022
Same author

Ancient Biosyntheses in an Oil Crop: Glucosinolate Profiles in <i>Limnanthes alba</i> and Its Relatives (Limnanthaceae, Brassicales).

Journal of agricultural and food chemistry·2022
Same author

Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin.

Plant methods·2021

Related Experiment Video

Updated: May 24, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
10:09

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Published on: March 15, 2017

Glucosinolate structures in evolution.

Niels Agerbirk1, Carl Erik Olsen

  • 1Section for Plant Biochemistry, Department of Plant Biology and Biotechnology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark. nia@life.ku.dk

Phytochemistry
|March 13, 2012
PubMed
Summary
This summary is machine-generated.

The number of known natural glucosinolates (GSLs) increased by 26 in the past decade, reaching 132 by 2011. Advanced methods like NMR spectroscopy are crucial for identifying novel GSL structures and understanding their evolution.

More Related Videos

13C6&#45;Glucose Labeling Associated with LC&#45;MS&#58; Identification of Plant Primary Organs in Secondary Metabolite Synthesis
04:32

13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

Published on: March 22, 2024

Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Related Experiment Videos

Last Updated: May 24, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
10:09

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)

Published on: March 15, 2017

13C6&#45;Glucose Labeling Associated with LC&#45;MS&#58; Identification of Plant Primary Organs in Secondary Metabolite Synthesis
04:32

13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

Published on: March 22, 2024

Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Area of Science:

  • Plant biochemistry
  • Natural product chemistry
  • Evolutionary biology

Background:

  • Over 100 natural glucosinolates (GSLs) were documented by 2000.
  • The past decade saw the elucidation of 26 new GSL structures, bringing the total to approximately 132 by 2011.
  • Many suggested GSL structures lack sufficient documentation, often due to insufficient NMR spectroscopic data.

Purpose of the Study:

  • To review GSL identification methods and highlight the importance of structure-sensitive techniques.
  • To present an example of qualitative GSL analysis, emphasizing NMR for structure elucidation.
  • To review recent investigations into GSL evolution and its implications.

Main Methods:

  • Review of GSL identification methodologies.
  • Qualitative GSL analysis including group separation, HPLC (intact and desulfated GSLs), UV, MS, NMR, and myrosinase susceptibility.
  • Analysis of GSL evolution based on species phylogeny.

Main Results:

  • By 2011, approximately 132 natural glucosinolates (GSLs) were documented, with 26 new structures identified in the preceding decade.
  • Fifteen of the newly documented GSLs were acyl derivatives, while others featured novel substitution patterns or functionalities.
  • Identification of a novel GSL, (R)-2-hydroxy-2-(3-hydroxyphenyl)ethylglucosinolate, was achieved using detailed analytical methods.
  • GSL profiles undergo regular evolution, suggesting natural selection for specific side chains and complex catabolism.

Conclusions:

  • NMR spectroscopy is vital for elucidating GSL structures, especially for minor compounds and hydrolysis products.
  • GSL evolution is an ongoing process, influenced by natural selection and potentially complex biochemical pathways.
  • Accurate GSL identification relies on authentic references and structure-sensitive detection methods like MS and NMR.