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Characterization of Neisseria meningitidis isolates from Egypt using multilocus sequence typing

John D Klena1, Momtaz O Wasfy, Rania A Nada

  • 1US Naval Medical Research Unit No. 3, Cairo, Egypt. irc4@cn.cdc.gov

Transactions of the Royal Society of Tropical Medicine and Hygiene
|March 13, 2012
PubMed

Insights

Meningitis surveillance in Egypt revealed serogroup B and A Neisseria meningitidis were dominant. Penicillin resistance necessitates alternative antibiotics like cephalosporins for effective treatment.

Area of Science:

  • Microbiology
  • Epidemiology
  • Infectious Diseases

Background:

  • Neisseria meningitidis causes bacterial meningitis, a significant public health concern.
  • Surveillance is crucial for understanding pathogen dynamics and guiding public health interventions, especially after vaccine introduction.

Purpose of the Study:

  • To characterize Neisseria meningitidis isolates from Egyptian meningitis cases (1998-2003).
  • To assess serogroup distribution, antibiotic resistance, and genetic lineages of N. meningitidis.

Main Methods:

  • Serogrouping of 67 N. meningitidis isolates.
  • Antibiotic sensitivity testing.
  • Multilocus sequence typing (MLST) for genetic analysis.

Main Results:

  • Serogroups B (50.7%) and A (34.3%) were predominant.
  • Intermediate penicillin resistance observed in 71% of isolates.
  • MLST identified recurrent lineages from Europe/Middle East and unique African/novel sequence types.

Conclusions:

  • The study highlights shifts in N. meningitidis epidemiology post-vaccine introduction in Egypt.
  • Increased penicillin resistance indicates a need to reconsider empirical treatment strategies.
  • Understanding genetic diversity is key for future meningitis control efforts.

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