Size comparison of ACL footprint and reconstructed auto graft

The purpose of this study was to compare the size of native anterior cruciate ligament (ACL) footprints and the size of commonly used auto grafts. The hypothesis was that the reconstructed graft size with auto grafts might be smaller than the native ACL footprint.

Fourteen non-paired human cadaver knees were used. The semitendinosus tendon (ST) and the gracilis (G) tendon were harvested and prepared for ACL grafts. Simulating an ST graft, the ST was cut in half. The bigger half was regarded as the antero-medial (AM) bundle, and the remaining half was regarded as the postero-lateral (PL) bundle. Simulating an ST-G graft, the bigger half of the ST and G were regarded as the AM bundle, and the smaller half of the ST was regarded as the PL bundle. Each graft diameter was measured, and the graft area was calculated. Simulating a rectangular bone-patella tendon-bone (BPTB) graft, a 10-mm wide BPTB graft was harvested and the area calculated. The ACL was carefully dissected, and the size of the femoral and tibial footprints was measured using Image J software (National Institution of Health).

The average areas of the ST, ST-G, and BPTB graft were 52.3 ± 7.3, 64.4 ± 9.2, and 32.7 ± 6.5 mm(2), respectively. The sizes of the native femoral and tibial ACL footprints were 85.4 ± 26.3 and 145.4 ± 39.8 mm(2), respectively. Only the ST-G graft showed no significant difference in graft size when compared with the femoral ACL footprint.

Only the ST-G auto graft was able to reproduce the native size of the ACL footprint on the femoral side. None of the auto grafts could reproduce the size of the tibial ACL footprint. For clinical relevance, ST-G graft is recommended in order to reproduce the native size of the ACL in anatomical ACL reconstruction with auto graft.