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Related Experiment Video

Updated: May 24, 2026

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
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Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

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Optimal image sample size for corneal nerve morphometry.

Dimitrios Vagenas1, Nicola Pritchard, Katie Edwards

  • 1Institute of Health and Biomedical Innovation and School of Optometry, Queensland University of Technology, Kelvin Grove, Queensland, Australia. dimitrios.vagenas@qut.edu.au

Optometry and Vision Science : Official Publication of the American Academy of Optometry
|March 13, 2012
PubMed
Summary
This summary is machine-generated.

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Determining the optimal number of corneal confocal microscopy images is crucial for accurate analysis of corneal nerve parameters. This study presents a method to establish the required sample size for reliable measurements of corneal nerve fiber length and branch density.

Area of Science:

  • Ophthalmology
  • Neuroscience
  • Biomedical Imaging

Background:

  • Corneal confocal microscopy is vital for assessing the corneal subbasal nerve plexus.
  • Current analysis often uses an arbitrary number of images, assuming representativeness.
  • Accurate quantification of corneal nerve parameters is essential for diagnosing and monitoring neurological conditions affecting the cornea.

Purpose of the Study:

  • To develop and present a technique for determining the optimal number of confocal microscopy images for accurate corneal nerve analysis.
  • To establish a method for quantifying the required sample size for reliable measurements of corneal nerve fiber length and branch density.

Main Methods:

  • Assessed all combinations of 2 to 16 non-overlapping (≤20%) central corneal confocal microscopy images from 20 subjects with type 2 diabetes.

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Last Updated: May 24, 2026

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
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Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

Published on: January 3, 2011

Quantification of Optic Nerve Cross Sectional Area on MRI: A Novel Protocol using Fiji Software
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  • Quantified corneal nerve fiber length and nerve branch density for each image combination.
  • Calculated mean ratios to compare measurements within and between subjects.

    • Main Results:

    • Eight randomly selected images (≤20% overlap) achieved 95% accuracy within 30% of the true mean for nerve branch density.
    • Five randomly selected images (≤20% overlap) achieved 80% accuracy within 13% of the true mean for corneal nerve fiber length.

    Conclusions:

    • The "sample combination analysis" provides a method to determine the necessary sample size for accurate quantification of corneal subbasal nerve parameters.
    • This technique can guide image selection in corneal confocal microscopy studies.
    • The methodology may be applicable to sample size determination in other biological studies.