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Recombinant DNA probe detecting Eperythrozoon suis in swine blood
R D Oberst1, S M Hall, R A Jasso
1Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
American Journal of Veterinary Research
|November 1, 1990
Summary
A new diagnostic probe, Eperythrozoon suis clone KSU-2, shows strong hybridization with E. suis in swine blood. This probe offers a specific method for detecting Eperythrozoon suis infections in pigs.
Area of Science:
- Molecular Biology
- Veterinary Diagnostics
- Genomics
Background:
- Eperythrozoon suis is an economically significant swine pathogen.
- Accurate and specific diagnostic tools are crucial for controlling E. suis infections.
Purpose of the Study:
- To develop and evaluate a DNA-based diagnostic probe for Eperythrozoon suis.
- To assess the specificity of the probe against other animal blood DNA.
Main Methods:
- Construction of a genomic library from E. suis DNA using lambda gt11.
- Identification and isolation of E. suis clone KSU-2.
- Hybridization experiments using the KSU-2 probe with DNA from various animal blood samples.
Main Results:
- The KSU-2 probe demonstrated strong hybridization with purified E. suis and blood from infected swine.
- No significant hybridization was observed with DNA from equine, bovine, canine, feline, or uninfected swine blood under stringent conditions.
Conclusions:
- E. suis clone KSU-2 is a highly specific DNA probe for the detection of Eperythrozoon suis.
- This probe has potential for use in the accurate diagnosis of swine eperythrozoonosis.