Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Integrins

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Selectins

Selectins

Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

Activation of Integrins

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bone marrow failure, somatic rescue by p53 inactivation, and enhanced leukemogenesis in germ line ERCC6L2 disease.

Blood·2026

Same author

Genetic resistance to leukemia.

Science (New York, N.Y.)·2026

Same author

The pathogenesis of therapy-related myeloid neoplasms from TP53-mutant clonal hematopoiesis.

Leukemia·2025

Same author

Succinate Dehydrogenase B (SDHB) Overexpression with Enzymatic Dysfunction Defines a Distinct Subtype of Undifferentiated Pleomorphic Sarcoma.

Cancer research communications·2025

Same author

Transcriptomic-Based Classification Identifies Prognostic Subtypes and Therapeutic Strategies in Soft Tissue Sarcomas.

Cancers·2025

Same author

Clonal Hematopoiesis: Impact on Health and Disease.

Hematological oncology·2025

Search research articles

Related Experiment Video

Updated: May 24, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Endothelial progenitor cells and integrins: adhesive needs.

Francisco Caiado¹, Sérgio Dias

¹Angiogenesis Laboratory, CIPM, Instituto Português de Oncologia Francisco Gentil, EPE, Lisboa, Portugal. sergidias@ipolisboa.min-saude.pt.

Fibrogenesis & Tissue Repair

|March 14, 2012

Summary

Endothelial progenitor cells (EPCs) form new blood vessels through postnatal vasculogenesis. Integrins on EPCs are crucial for their mobilization, homing, and differentiation, offering therapeutic targets for enhancing EPC function.

More Related Videos

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

Quantitation of Endothelial Cell Adhesiveness In Vitro

Quantitation of Endothelial Cell Adhesiveness In Vitro

Published on: June 18, 2015

Related Experiment Videos

Last Updated: May 24, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

Quantitation of Endothelial Cell Adhesiveness In Vitro

Quantitation of Endothelial Cell Adhesiveness In Vitro

Published on: June 18, 2015

Area of Science:

Cell Biology
Vascular Biology
Regenerative Medicine

Background:

Endothelial progenitor cells (EPCs) contribute to new blood vessel formation (vasculogenesis) in adults.
EPCs undergo a four-step process: mobilization, homing, invasion/migration, and differentiation or paracrine signaling.
EPC function is influenced by interactions within various physiological compartments.

Purpose of the Study:

To review the role of integrins in regulating EPC function during postnatal vasculogenesis.
To highlight how specific integrin subunits impact different stages of EPC biology.
To explore the therapeutic potential of manipulating integrin-mediated EPC interactions.

Main Methods:

Review of existing literature on EPCs and integrin function.
Summary of studies investigating integrin-ligand interactions in EPCs.
Analysis of data linking integrin expression to EPC mobilization, homing, invasion, differentiation, and signaling.

Main Results:

Integrins are key regulators of EPC function throughout postnatal vasculogenesis.
Specific integrins like α4β1 control EPC mobilization, while α5β1, α6β1, αvβ3, and αvβ5 regulate homing, invasion, differentiation, and paracrine activity.
β2 integrins are critical for EPC transendothelial migration.

Conclusions:

Integrins are essential molecular determinants of EPC biology.
Targeting integrin-mediated interactions offers a strategy to enhance EPC therapeutic potential.
Further research into tissue-specific manipulation of EPC integrins could advance clinical applications.