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Expression of mammalian renal transporters in Xenopus laevis oocytes
M J Coady1, A M Pajor, E M Toloza
1Department of Physiology, UCLA School of Medicine 90024-1751.
Archives of Biochemistry and Biophysics
|November 15, 1990
Abstract:
We have injected mRNA from rabbit renal cortex into Xenopus oocytes and demonstrated the expression of renal carriers for Na(+)-independent transport of L-phenylalanine and L-lysine and Na(+)-dependent transport of L-alanine and succinate. Maximal expression of renal amino acid transporters occurred 6-8 days following mRNA injection. The proteins responsible for transport of these four substrates were translated from mRNAs which are between 1.5 and 3.0 kb. This information serves as a starting point for expression cloning of these transport proteins.