Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Bacterial Phylum Proteobacteria01:26

Bacterial Phylum Proteobacteria

Proteobacteria, one of the largest and most diverse bacterial phyla, encompasses a wide range of Gram-negative bacteria distinguished by their outer membrane composed of lipopolysaccharides. These microorganisms exhibit various metabolic capabilities, including phototrophy, chemolithotrophy, and heterotrophy, and thrive in diverse environments from soil to aquatic systems and host-associated niches. The phylum is divided into six classes: Alphaproteobacteria, Betaproteobacteria,...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Gram-negative Bacterial Protein Secretion Systems01:17

Gram-negative Bacterial Protein Secretion Systems

Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pathotypes and Drug Susceptibility of Escherichia coli Isolated from Companion Dogs in Japan.

Japanese journal of infectious diseases·2020
Same author

Novel Sequence Type in Bacillus cereus Strains Associated with Nosocomial Infections and Bacteremia, Japan.

Emerging infectious diseases·2019
Same author

<i>Corynebacterium striatum</i> Bacteremia Associated with a Catheter-Related Blood Stream Infection.

Case reports in infectious diseases·2017
Same author

Effect of vitamin K2 on the development of stress-induced osteopenia in a growing senescence-accelerated mouse prone 6 strain.

Experimental and therapeutic medicine·2015
Same author

[Concern Regarding a Mumps Epidemic Through Antibody Screening in College Students].

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases·2015
Same author

Molecular epidemiological characterization of uropathogenic escherichia coli from an outpatient urology clinic in rural Japan.

Journal of clinical microbiology·2014

Related Experiment Video

Updated: May 24, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

[KPC carbapenemase producing Gram-negative bacteria].

Kunikazu Yamane1

  • 1Department of Public Health, Kawasaki Medical School.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|March 15, 2012
PubMed
Summary
This summary is machine-generated.

Carbapenem-resistant Gram-negative bacteria are a global threat. In Japan, bla(IMP-1) is more common than bla(KPC), necessitating new detection methods for KPC-producing bacteria.

More Related Videos

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

Related Experiment Videos

Last Updated: May 24, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Context:

  • Carbapenems are critical last-resort antibiotics for Gram-negative bacterial infections.
  • Carbapenem-resistant Enterobacteriaceae (CRE), including KPC-producing strains, are increasingly prevalent worldwide.
  • A 2010 nationwide surveillance study in Japan investigated multi-drug resistant Enterobacteriaceae.

Purpose:

  • To determine the prevalence of carbapenemase genes, specifically bla(KPC) and bla(IMP-1), in multi-drug resistant Enterobacteriaceae in Japan.
  • To compare the epidemic carbapenemase in Japan with global trends.

Summary:

  • The study identified two bla(KPC)-harboring Klebsiella pneumoniae strains among 153 multi-drug resistant Enterobacteriaceae.
  • Notably, 72 strains harbored the bla(IMP-1) gene, indicating its higher prevalence in Japan.
  • This surveillance revealed bla(IMP-1) as the predominant carbapenemase in Japan, not bla(KPC).

Impact:

  • The findings highlight that bla(IMP-1) is the most epidemic carbapenemase in Japan, contrasting with the global rise of bla(KPC).
  • The rarity of bla(KPC) strains in Japan poses a challenge for current detection methods.
  • There is a critical need for developing effective screening methods for KPC-producing Gram-negative bacteria within the Japanese healthcare system.