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Association between single nucleotide polymorphisms in p53 and abortion in Thoroughbred mares
Priscila Marques Moura de Leon1, Vinicus Farias Campos, Helena Strelow Thurow
1Laboratório de Embriologia Molecular e Transgênese, Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Veterinary Journal (London, England : 1997)
|March 15, 2012
Summary
The p53 gene
Area of Science:
- Equine genetics
- Animal reproduction
- Molecular biology
Background:
- Single nucleotide polymorphisms (SNPs) in the p53 gene are well-documented in human studies.
- The p53 gene's role in cellular processes suggests potential involvement in reproductive functions.
- Understanding genetic variations in equine populations is crucial for improving breeding outcomes.
Purpose of the Study:
- To investigate the frequency of the Arg/Pro single nucleotide polymorphism (SNP) in the p53 gene among Thoroughbred mares in Brazil.
- To establish correlations between different p53 genotypes and key reproductive performance indicators, specifically abortion rates.
- To explore the genetic underpinnings of reproductive success in equine breeding.
Main Methods:
- Genotyping of 105 Thoroughbred mares using PCR-restriction fragment length polymorphism (PCR-RFLP) on blood samples.
- Confirmation of single nucleotide polymorphism (SNP) detection through DNA sequencing.
- Statistical analysis, including logistic regression, to assess the association between p53 genotypes and abortion incidence.
Main Results:
- The allele frequency for the p53 gene (codon 72, exon 4) in Thoroughbred mares was determined: Arg/Pro (73.3%), Arg/Arg (17.1%), and Pro/Pro (9.6%).
- A significant association was found between the Arg/Pro genotype and an increased risk of abortion (P=0.02).
- Conversely, mares with the Pro/Pro genotype exhibited a reduced probability of abortion (P<0.05), with a significant dominance effect for abortion (P=0.044; OR=7.94).
Conclusions:
- The p53 gene Arg/Pro single nucleotide polymorphism (SNP) is prevalent in Thoroughbred mares in Brazil.
- Specific p53 genotypes are significantly associated with abortion rates in equine reproduction.
- The p53 gene likely plays a role in the reproductive success of horses, warranting further investigation.
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