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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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Related Experiment Video

Updated: May 24, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Functionalized electrospun nanofibers as bioseparators in microfluidic systems.

Lauren Matlock-Colangelo1, Daehwan Cho, Christine L Pitner

  • 1Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY, USA.

Lab on a Chip
|March 15, 2012
PubMed
Summary

Functionalized nanofibers in microfluidic devices act as bioseparators, enabling on-chip isolation and concentration of nanoparticles. This innovation simplifies lab-on-a-chip sample preparation for broader applications.

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Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
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Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
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Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

Related Experiment Videos

Last Updated: May 24, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape
07:38

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

Area of Science:

  • Biomaterials Engineering
  • Microfluidics
  • Nanotechnology

Background:

  • Microfluidic devices offer miniaturized platforms for biological sample analysis.
  • Efficient on-chip bioseparation is crucial for sample preparation in lab-on-a-chip systems.
  • Integrating functional materials into microfluidics remains a challenge.

Purpose of the Study:

  • To develop and demonstrate functionalized electrospun nanofibers as on-chip bioseparators in microfluidic devices.
  • To investigate the use of poly(vinyl alcohol) (PVA) nanofibers for nanoparticle separation.
  • To explore methods for selective binding and release of targets within microfluidic channels.

Main Methods:

  • Electrospinning of poly(vinyl alcohol) (PVA) nanofibers onto microelectrodes.
  • Integration of nanofiber-coated electrodes into poly(methyl methacrylate) (PMMA) microfluidic devices via UV-assisted thermal bonding.
  • Functionalization of PVA nanofibers with poly(hexadimethrine bromide) (polybrene) for positive charge and Poly(methyl vinyl ether-alt-maleic anhydride) (POLY(MVE/MA)) for negative charge.
  • Liposome capture and retention studies using confocal fluorescence microscopy.
  • Selective release of captured liposomes using a pH-adjusted buffer solution.

Main Results:

  • Positively charged polybrene-functionalized PVA nanofibers successfully captured negatively charged liposomes.
  • Negatively charged POLY(MVE/MA)-functionalized nanofibers repelled liposomes.
  • A broad optimal range of nanofiber mat thickness was identified for reliable liposome retention.
  • Captured liposomes were selectively released by altering the nanofiber surface charge using a pH 9 buffer solution.

Conclusions:

  • Functionalized electrospun nanofibers serve as effective on-chip bioseparators in microfluidic devices.
  • This approach enables crucial sample preparation steps like isolation and concentration within lab-on-a-chip systems.
  • The integration of functional surfaces into microfluidics is significantly advanced, expanding the capabilities of lab-on-a-chip devices.