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Tissue Homogenization and Cell Lysis

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

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Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Strategies for tissue and organ decellularization.

Thomas W Gilbert¹

¹Cardiothoracic Surgery, and Bioengineering, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15224, USA. gilberttw@upmc.edu

Journal of Cellular Biochemistry

|March 15, 2012

Summary

Decellularized tissues and organs are key in regenerative medicine. Different decellularization methods are used for tissues and whole organs, impacting the extracellular matrix and host response.

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Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Decellularized tissues are widely used in regenerative medicine and tissue engineering.
Recent advancements include the use of decellularized organs for organ engineering.
Significant differences exist in decellularization protocols for simple tissues versus whole organs.

Purpose of the Study:

To describe common decellularization methods for surgical mesh and whole organs.
To analyze how these decellularization processes influence the extracellular matrix.
To evaluate the impact of decellularization on the host response to scaffolds.

Main Methods:

Review of commonly used decellularization techniques for surgical mesh materials.
Review of commonly used decellularization techniques for whole organs.
Analysis of the effects of decellularization on the extracellular matrix composition and structure.
Assessment of host responses to decellularized scaffolds.

Main Results:

Decellularization protocols vary significantly between simple tissues and complex organs.
The choice of decellularization method directly affects the integrity and composition of the extracellular matrix.
Different decellularization processes elicit distinct host responses to the resulting scaffolds.

Conclusions:

Understanding decellularization methods is crucial for successful tissue and organ engineering.
Optimizing decellularization protocols can enhance scaffold properties and improve regenerative outcomes.
Further research is needed to fully elucidate the relationship between decellularization, matrix properties, and host integration.