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A boradiazaindacene-based turn-on fluorescent probe for cyanide detection in aqueous media

Chi-Hwa Lee1, Hee-Jae Yoon, Jeong-Soo Shim

  • 1Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemungu, Seoul 120-749, Korea.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|March 15, 2012
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...