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Related Concept Videos

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Related Experiment Video

Updated: May 24, 2026

Laboratory Production of Biofuels and Biochemicals from a Rapeseed Oil through Catalytic Cracking Conversion
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Published on: September 2, 2016

Lipase-catalyzed interesterification in packed bed reactor using 2 different temperatures.

Mi-Hwa Chae1, Hye-Kyung Park, Kwang-Il Kwon

  • 1Dept. of Food & Nutrition, Korea Univ., Seoul, 136-703, Republic of Korea.

Journal of Food Science
|March 16, 2012
PubMed
Summary

A stepwise temperature protocol using immobilized lipase (Lipozyme TL IM) maintained enzyme activity during fat interesterification. This method preserved fat properties while significantly enhancing enzyme stability over 50 cycles compared to constant temperatures.

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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
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11:33

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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
13:00

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Published on: April 4, 2014

Area of Science:

  • Biocatalysis
  • Food Chemistry
  • Enzyme Engineering

Background:

  • Lipase-catalyzed interesterification is crucial for modifying fat properties.
  • Optimizing reaction conditions, such as temperature, is key to improving enzyme efficiency and stability.
  • High oleic sunflower oil and fully hydrogenated soybean oil are common substrates for fat modification.

Purpose of the Study:

  • To investigate the effect of a stepwise temperature protocol on lipase-catalyzed interesterification.
  • To compare the performance of a stepwise temperature protocol with a constant temperature protocol.
  • To evaluate the impact on enzyme residual activity, conversion degree, triacylglycerol profile, and solid fat content.

Main Methods:

  • Lipase-catalyzed interesterification using immobilized Thermomyces lanuginosus lipase (Lipozyme TL IM).
  • Packed bed reactor utilized for continuous process.
  • Comparison of a constant 70 °C protocol with a stepwise protocol (70 °C for 9 min, then 60 °C).

Main Results:

  • No significant differences in conversion degree, triacylglycerol profile, or solid fat content between protocols.
  • Stepwise temperature protocol resulted in significantly higher overall residual enzyme activities after 50 cycles.
  • Melting point analysis suggested an optimal lower reaction temperature after initial incubation.

Conclusions:

  • A stepwise temperature protocol can enhance the operational stability of immobilized lipases in fat interesterification.
  • This approach maintains product quality while improving enzyme longevity.
  • Enzyme immobilization and process optimization are critical for sustainable biocatalysis in the food industry.