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Preparation, Purification, and Use of Fatty Acid-containing Liposomes
Published on: February 9, 2018
Process improvement for semipurified oleosomes on a pilot-plant scale
Virginie N Kapchie1, Catherine C Hauck, Hui Wang
1Dept. of Food Science and Human Nutrition, Center for Crops Utilization Research, Iowa State Univ., Ames, IA 50011, USA. vkapchie@iastate.edu
Journal of Food Science
|March 16, 2012
Summary
An improved process efficiently recovers intact soybean oleosomes, yielding 91% oil recovery. This method also isolates valuable proteins and phytochemicals like isoflavones and saponins.
Area of Science:
- Agricultural Chemistry
- Biotechnology
- Food Science
Background:
- Soybean oleosomes are complex oil bodies containing proteins and bioactive compounds.
- Efficient extraction of intact oleosomes is crucial for utilizing their components.
Purpose of the Study:
- To develop and evaluate an improved pilot-plant scale process for semipurified oleosome extraction from soybeans.
- To investigate the fractionation, protein content, and recovery of oleosins, isoflavones, and saponins.
Main Methods:
- Utilized continuous centrifugation in a three-phase decanter with slurry recirculation for oleosome recovery.
- Analyzed oleosome, aqueous supernatant, and residue fractions for oil, protein, oleosin, isoflavone, and saponin content.
- Washed semipurified oleosomes to recover associated storage proteins.
Main Results:
- Achieved 91%±1% soybean oil recovery as intact oleosomes within 8 hours.
- Identified oleosins at 17 and 18 kDa.
- Found significant recovery of isoflavones (82 mole%) and saponins (63 mole%) in oleosome and aqueous supernatant fractions.
Conclusions:
- The improved process is efficient for pilot-plant scale oleosome extraction, yielding high oil recovery.
- The process effectively fractionates soybean proteins and recovers valuable phytochemicals, presenting opportunities for value-added products.
