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[DNA extraction from body fluids of cadavers]
E Josephi1, U Braito, E Tutsch-Bauer
1Institut für Rechtsmedizin, Universität München.
Beitrage Zur Gerichtlichen Medizin
|January 1, 1990
Summary
Human DNA can be detected in various body fluids from deceased individuals. Sex determination is possible using sex-specific DNA probes on these samples.
Area of Science:
- Forensic Science
- Molecular Biology
- Genetics
Context:
- Analysis of post-mortem biological samples is crucial in forensic investigations.
- Identifying human DNA in diverse body fluids aids in identification and legal proceedings.
- Standard DNA profiling methods can be challenging with limited or degraded samples.
Purpose:
- To investigate the presence and origin of DNA in various post-mortem body fluids.
- To evaluate the utility of dot-blot hybridization for human DNA detection.
- To assess the feasibility of sex determination from these biological samples using sex-specific DNA probes.
Summary:
- DNA was isolated from multiple body fluids (ascites, bile, liquor, pleural/pericardial fluid, synovial fluid, urine, vitreous humor) of sixteen deceased individuals.
- Dot-blot hybridization using total human DNA as a probe confirmed the presence of human DNA in these fluids.
- Rehybridization with a sex-specific DNA probe (Y) enabled successful sex determination, indicating the potential of this method.
Impact:
- Establishes a method for confirming human origin of biological samples from diverse post-mortem fluids.
- Provides a forensic tool for sex determination from challenging biological evidence.
- Enhances the capabilities of forensic analysis in identifying individuals and determining biological characteristics from deceased persons.