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Related Experiment Video

Updated: May 24, 2026

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Published on: April 28, 2017

Scattered light fluorescence microscopy in three dimensions.

Giulia Ghielmetti¹, Christof M Aegerter

¹Physik-Institut, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

|March 16, 2012

Summary

This summary is machine-generated.

Researchers developed a 3D imaging technique for fluorescent structures behind turbid layers. This method uses wave-front shaping and the memory effect, enabling deeper visualization in scattering media like biological tissues.

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Area of Science:

Optics
Biomedical Imaging
Microscopy

Background:

Imaging through scattering media is challenging due to light distortion.
Wave-front shaping and the memory effect allow focusing through turbid layers.
Previous methods were limited to 2D imaging.

Purpose of the Study:

To extend 2D imaging through turbid layers to 3D.
To enable volumetric imaging of fluorescent structures behind scattering media.
To demonstrate a new capability for deep-tissue fluorescence microscopy.

Main Methods:

Wave-front shaping was employed to control light propagation.
The optical memory effect was utilized for focusing.
A parabolic phase shift was added to enable z-axis scanning.
Scattered light fluorescence microscopy was used for imaging.

Main Results:

Successfully demonstrated 3D imaging of fluorescent nano-beads.
Achieved diffraction-limited resolution behind a turbid layer (>10 mean free paths).
Enabled scanning of a three-dimensional volume, not just a 2D plane.

Conclusions:

The addition of a parabolic phase shift enables 3D scanning in scattered light microscopy.
This technique significantly expands the possibilities for imaging deep within scattering biological tissues.
The study highlights the potential and limitations of z-direction scanning compared to x-y plane scanning.