Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CeraMIRScan: Mid-infrared OCT Scan Dataset for Ceramic Quality Assessment.

Scientific data·2026
Same author

Mechanistic Insights into Per- and Polyfluoroalkyl Substance (PFAS) Photolysis under Intensified Simulated Solar Light.

Environmental science & technology·2026
Same author

Ultra-low-noise supercontinuum in normal-dispersion ZBLAN fibers pumped at 1.85 µm.

Optics letters·2026
Same author

The primary near-UV photochemistry of aqueous pyruvic acid.

Physical chemistry chemical physics : PCCP·2026
Same author

The primary deep-UV photochemistry of aqueous fumarate and maleate.

Physical chemistry chemical physics : PCCP·2026
Same author

Exploiting wave breaking asymmetry for broadband cascaded ANDi-supercontinuum generation.

Optics letters·2025

Related Experiment Video

Updated: May 24, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

IR microscopy utilizing intense supercontinuum light source.

Sune Dupont1, Christian Petersen, Jan Thøgersen

  • 1Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark.

Optics Express
|March 16, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel infrared supercontinuum light source for high-resolution hyperspectral infrared microscopy. This breakthrough enables detailed, contact-free chemical imaging with unprecedented molecular specificity.

More Related Videos

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Related Experiment Videos

Last Updated: May 24, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

  • Spectroscopy and Microscopy
  • Optical Engineering
  • Materials Science

Background:

  • Combining infrared spectroscopy with microscopy offers molecular specificity.
  • Achieving high resolution in infrared microspectroscopy has been a long-standing research goal.

Purpose of the Study:

  • To demonstrate an optical fiber-based infrared supercontinuum as a novel light source for microspectroscopy.
  • To enable high-resolution, contact-free hyperspectral infrared microscopy.

Main Methods:

  • Generation of broadband infrared supercontinuum light from an optical fiber.
  • Development of a hyperspectral infrared microscope utilizing the supercontinuum source.
  • Imaging of an oil/water sample to demonstrate system performance.

Main Results:

  • The supercontinuum source provides high brightness across the 1400-4000 nm infrared range.
  • The developed microscope achieves 20 μm resolution.
  • Contact-free hyperspectral imaging capability was successfully demonstrated.

Conclusions:

  • Infrared supercontinuum from optical fibers is a viable and promising light source for advanced infrared microspectroscopy.
  • This technology facilitates high-resolution, contact-free chemical imaging.
  • The system shows potential for various applications requiring detailed molecular analysis.