Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Refractive Index Mapping below the Diffraction Limit via Single Molecule Localization Microscopy.

ACS nano·2025
Same author

Coded wavefront sensing for video-rate quantitative phase imaging and tomography: validation with digital holographic microscopy.

Optics express·2025
Same author

Joint estimation of point spread function and molecule positions in SMLM informed from multiple planes.

Biomedical optics express·2025
Same author

Confocal Raman Microscopy with Adaptive Optics.

ACS photonics·2025
Same author

Quantitative phase imaging with optical differentiation by spatially variable amplitude filters.

Optics letters·2025
Same author

Low cross-talk optical addressing of trapped-ion qubits using a novel integrated photonic chip.

Light, science & applications·2024
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Quantitative single-shot imaging of complex objects using phase retrieval with a designed periphery.

Alexander Jesacher1, Walter Harm, Stefan Bernet

  • 1Division of Biomedical Physics, Innsbruck Medical University, A-6020 Innsbruck, Austria. alexander.jesacher@i-med.ac.at

Optics Express
|March 16, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a novel phase object method to enhance phase retrieval algorithms for optical imaging. The technique improves accuracy and speed for measuring transmission and optical thickness from a single image.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Related Experiment Videos

Last Updated: May 24, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Area of Science:

  • Optics
  • Image Processing
  • Metrology

Background:

  • Quantitative imaging often requires measuring an object's transmission and optical thickness.
  • Phase retrieval algorithms offer a path to achieve this from intensity data.
  • Existing methods can suffer from slow convergence and artifacts like the twin-image.

Purpose of the Study:

  • To develop a more accurate and efficient phase retrieval method for optical imaging.
  • To enable quantitative imaging using a single intensity recording.
  • To suppress the twin-image artifact common in holographic techniques.

Main Methods:

  • Introduction of a specially designed phase object into the specimen plane.
  • Utilizing this phase object as a constraint within the phase retrieval algorithm.
  • Recording the diffraction pattern in the camera plane without lenses.

Main Results:

  • Significantly improved performance of phase retrieval algorithms.
  • Faster algorithm convergence and enhanced final accuracy.
  • Effective suppression of the twin-image artifact.
  • Quantitative imaging accuracy comparable to phase-stepping interferometry.

Conclusions:

  • The proposed phase object method offers a robust solution for accurate quantitative imaging.
  • This technique simplifies the imaging process by requiring only a single intensity recording.
  • The method provides a valuable advancement for optical imaging research and applications.