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Inhibition of TGF alpha-induced second messengers by anti-EGF receptor antibody-425

U Murthy1, D J Rieman, U Rodeck

  • 1Department of Cell Sciences, SmithKline Beecham, King of Prussia, PA 19406.

Biochemical and Biophysical Research Communications
|October 30, 1990
PubMed

Monoclonal antibody 425 binds to a protein epitope of the human EGF receptor and blocks EGF dependent functions such as EGF receptor phosphorylation and mitogenesis (1). We now show that MAb 425 blocks TGF alpha-induced second messenger signals, namely inositol 1,4,5 triphosphate and Ca2+ in two carcinoma cell lines, A 431 and SW-948. In this study we have further characterized the specificity of this antibody for inhibiting TGF alpha induced mitogenesis in MRC-5, a EGF-receptor expressing fibroblast cell line.

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