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Updated: May 24, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

Plant genetics for forensic applications.

David N Zaya¹, Mary V Ashley

¹Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 16, 2012

Summary

Plant DNA fingerprinting offers new forensic tools for crime scene analysis and identifying commercial product origins. This review covers DNA-based forensic botany applications and a leaf genotyping protocol for linking suspects to evidence.

Area of Science:

Forensic Botany
Molecular Biology
Genetics

Background:

Plant DNA fingerprinting is increasingly used in forensic science.
Botanical evidence analysis aids crime scene investigations and commercial product sourcing.
Illicit drug trade investigations can benefit from plant DNA analysis.

Purpose of the Study:

To review current and potential applications of DNA-based forensic botany.
To present a protocol for microsatellite genotyping of leaf material for forensic use.

Main Methods:

Review of scientific literature on DNA-based forensic botany.
Development and description of a microsatellite genotyping protocol for plant leaf samples.

Main Results:

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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

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Published on: February 20, 2012

Identified diverse forensic applications of plant DNA analysis.
Detailed a microsatellite genotyping protocol for linking botanical evidence to suspects or scenes.

Conclusions:

DNA-based forensic botany provides powerful tools for legal investigations.
The presented protocol facilitates the use of plant material in forensic casework.