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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Genome Annotation and Assembly

Genome Annotation and Assembly

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Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Evolutionary Relationships through Genome Comparisons

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Related Experiment Video

Updated: May 24, 2026

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

SEQanswers: an open access community for collaboratively decoding genomes.

Jing-Woei Li¹, Robert Schmieder, R Matthew Ward

¹School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR.

Bioinformatics (Oxford, England)

|March 16, 2012

Summary

This summary is machine-generated.

High-throughput sequencing generates vast genomic data, requiring rapid, interdisciplinary collaboration. SEQanswers offers a real-time knowledge-sharing platform for sequencing and analysis, fostering scientific advancement.

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Last Updated: May 24, 2026

Novel Sequence Discovery by Subtractive Genomics

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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

Published on: May 9, 2017

Area of Science:

Genomics
Bioinformatics
Computational Biology

Background:

High-throughput sequencing (HTS) affordability drives increased genomic data use in research.
Rapid advancements in sequencing technology necessitate efficient knowledge exchange.

Purpose of the Study:

To introduce SEQanswers as a real-time knowledge-sharing resource.
To facilitate interdisciplinary discussion on sequencing and analysis.

Main Methods:

Community-driven platform for sharing experimental and computational insights.
Active membership includes analysis tool developers.

Main Results:

SEQanswers has over 4000 active members.
~40 peer-reviewed publications have referenced the platform.

Conclusions:

SEQanswers addresses the need for fast, collaborative discussion in genomics research.
The platform supports both experimental and computational aspects of sequencing and analysis.